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. 2022 Apr 14;14(4):862. doi: 10.3390/pharmaceutics14040862

Figure 2.

Figure 2

Biological activity of trehalose, conjugates 1a and 1b and NAs 5a and 5b. We assessed LC3B conversion upon compound treatment by Western blotting. (A) Evaluation of autophagy induction in HeLa cells treated for 24 h with trehalose, 1b and 1a. Upon treatment, we analyzed tubulin, LC3BI and II levels by Western blotting. (B) Evaluation of autophagy induction in HeLa cells treated for 24 h with trehalose and 5b. Upon treatment, we analyzed α-tubulin, LC3BI and II levels by Western blotting. (C) Evaluation of autophagy induction in HeLa cells treated for 24 h with trehalose and 5a. Upon treatment, we analyzed α-tubulin, LC3BI and II levels by Western blotting.