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. 2022 Apr 17;14(4):833. doi: 10.3390/v14040833

Figure 1.

Figure 1

Screening of highly active sgRNAs for detection of PEDV, TGEV, PDCoV, and SADS-CoV. Identification of the highly active sgRNA targeting PEDV ORF3 gene (A), TGEV N gene (B), PDCoV N gene (C), and SADS-CoV N gene (D) by CRISPR/Cas12a cleavage assay; DNA template represents the amplification fragment of ORF3 or N gene as the template; ssDNA activator represents the complementary single-stranded DNA of the sgRNA. No excitation light stands for white light. PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; PDCoV, porcine deltacoronavirus; SADS-CoV, swine acute diarrhea syndrome coronavirus.