Figure 2.

Sensitivity of CRISPR/Cas12a combined with LAMP assay for portable detection of PEDV, PDCoV, TGEV, and SADS-CoV. (A,C,E,G) Colorimetric/fluorescence signals from a series of 10-fold dilutions of plasmid DNA by CRISPR/Cas12a coupled with LAMP assay. (B,D,F,H) Sensitivity of the CRISPR/Cas12a coupled with LAMP assay quantified by a multi-functional microplate reader (n = 3). No excitation light stands for white light; PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; PDCoV, porcine deltacoronavirus; SADS-CoV, swine acute diarrhea syndrome coronavirus; NC, negative control. Data are represented as mean ± SEM.