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. 2022 Apr 17;14(4):833. doi: 10.3390/v14040833

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Specificity of LAMP-Cas12a assay for detection of four porcine coronaviruses. (A) The agarose gel electrophoresis for detection of specific LAMP amplicons; Lane M: 2000 DNA Ladder; bp: base pairs; Lane 1: PEDV-ORF3 plasmid as DNA template; Lane 2: TGEV-N plasmid as DNA template; Lane 3: PDCoV-N plasmid as DNA template; Lane 4: SADS-CoV-N plasmid as DNA template; Lane 5: non-template control (NTC). (B) The colorimetric/fluorescence signal detection under the blue (470 nM) and UV light; LAMP-amplified products of PEDV-ORF3 plasmid (1), TGEV-N plasmid (2), PDCoV-N plasmid (3), SADS-CoV-N plasmid (4), non-template control (5). No excitation light stands for white light; PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; PDCoV, porcine deltacoronavirus; SADS-CoV, swine acute diarrhea syndrome coronavirus.