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. 2022 Apr 17;14(4):833. doi: 10.3390/v14040833

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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One-tube detection of four porcine coronaviruses in clinical samples based on CRISPR/Cas12a combined with RT-LAMP assay. (A) Diagram of principle of the contamination-free one-tube CRISPR/Cas12a combined with RT-LAMP assay detection of clinical samples. (B) Colorimetric/fluorescence readout of four porcine CoVs by one-tube multiplex RT-LAMP-Cas12a assay. CRISPR, clustered regularly interspaced short palindromic repeats; RT-LAMP, reverse transcription-loop-mediated isothermal amplification; No excitation light stands for white light; PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; PDCoV, porcine deltacoronavirus; SADS-CoV, swine acute diarrhea syndrome coronavirus; NC, negative control.