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. 2022 Apr 7;27(8):2385. doi: 10.3390/molecules27082385

Figure 1.

Figure 1

SDS-PAGE of Ageritin digested by proteases. Panel (A): 5 µg of Ageritin (A, negative control); lanes 1, 2 and 3, trypsin inhibited after 10 min, 30 min and 60 min, respectively. Panel (B): 5 µg of Ageritin (A, negative control); lanes 1, 2 and 3, chymotrypsin inhibited after 10 min, 30 min and 60 min, respectively. Insert of both panel a and b, densitometric analysis. Panel (C): 5 µg of Ageritin (A, negative control); lanes 1, 2 and 3, pepsin inhibited after 10 min, 30 min and 60 min, respectively. M, molecular weight markers; denatured SDS-PAGE was carried out in 15% polyacrylamide separating gel in reducing conditions (β-mercaptoethanol).