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. 2022 Apr 22;17(4):e0266601. doi: 10.1371/journal.pone.0266601

Fig 2. CPI-613 in combination with galloflavin inhibits pancreatic cancer cell proliferation and induces cell death.

Fig 2

(A) Proliferation of 6606PDA cells after cultivating them in CPI-613 (100–400 μM) with and without 30 μM galloflavin (GAL; dotted lines indicate mean value of absorbance for control treated cells, either with high or low DMSO concentrations). (B) Analysis of proliferation by BrdU-ELISA, (C) or quantification of cell death by trypan blue assay, after cultivating 6606PDA cells 24 h in control medium with the respective amount of the vehicle DMSO (C), medium supplemented with 300 μM CPI-613 (CPI), 30 μM galloflavin (GAL) or the combination of both drugs (CPI + GAL). (D-E) Apoptosis was assessed by analyzing the cleavage of caspase 3 (caspase-3) and PARP via western blot, after treating the cells with medium supplemented with DMSO (C), 300 μM CPI-613, 30 μM galloflavin or the combination for 24 h respectively (the bands of the cleaved proteins are indicated by black arrows). Statistics were performed with ANOVA on Ranks Holm-Sidak method for multi comparison, significant differences: * p ≤ 0.004; Independent experiments: A-B: n = 8–10, C: n = 19, D-E: n = 2–3.