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. 2022 Mar 29;14(4):712. doi: 10.3390/v14040712

Figure 1.

Figure 1

Isolation and identification of PRV-GD and PRV-JM isolates. (A), The cytopathic effects (CPEs) of PK-15 cells infected by PRV-GD and PRV-JM for 24 h. The CPEs of PK-15 cells infected with PRV-TJ strain were considered as the positive control. The arrowhead indicates the CPEs observed of PRV-GD and PRV-JM-inoculated PK-15 cells. The CPEs were characterized with rounded and floated cells. (B), PCR amplification of PRV gE (870 bp) and gB (790 bp) fragments from PRV-GD and PRV-JM-inoculated PK-15 cells. DNA+ was the positive control and PCR− was the negative control during PCR amplification. (C), One-step growth curves and gB protein assessment of 3 PRV strains on PK-15 cells at a multiplicity of infection of 0.1. (D), Phylogenetic tree based on genomic nucleotide sequence. PRV-GD and PRV-JM isolates in this work were indicated with a red dot. The phylogenetic tree was constructed by the adjacency method in MEGA 7 (http://www.megasoftware.net, accessed on 14 July 2021).