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. 2022 Apr 15;14(8):1651. doi: 10.3390/nu14081651

Figure 1.

Figure 1

Mouse body weights and plasma TMAO levels in vivo. Apoe−/− mice were transfected with cholesterol ester transfer protein (CETP)-AAV at 7–8 weeks of age prior to choline supplementation. Animals were assigned to the indicated treatment groups at 9–10 weeks of age and fed diets for 16 weeks. (A) Mouse body weights for all treatments were evaluated to monitor overall health. (B) Plasma levels of trimethylamine N-oxide (TMAO) were measured at baseline, as well as 8 and 16 weeks after treatment. Data are presented as mean ± SEM (n = 10–12 mice/group). One-factor ANOVA test was used to analyse normally distributed data, and a Kruskal–Wallis test was performed for non-normally distributed data. Post hoc Tukey’s honestly significant difference test (HSD) pairwise comparisons were conducted to identify statistical differences between the treatment groups when a significant main effect was found. Means that do not share a letter are considered significantly different; p < 0.05.