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Effects of lipid formulations of AMB on TNF-α production. Macrophages (105 per well) were cultured with IFN-γ (20 IU/ml) and various concentrations and formulations of AMB. After an activation phase of 5 h, TNF-α production was determined as described in Materials and Methods. (A) Liposomes. Filled bars, AMB; open bars, AmBisome, cross-hatched bars, ampholiposomes. (B) Complexes. Filled bars, AMB; open bars, Abelcet; dotted bars, LC-AMB; cross-hatched bars, Amphotec.
