TABLE 3.
Influence of the lipid on the activation of murine peritoneal macrophagesa
| AMB formulation | AMB concn (μg/ml) | NO2− concn (μmol/liter) | TNF-α concn (pg/ml) |
|---|---|---|---|
| AMBc | 1.0 | 25 ± 4 | 297 ± 12 |
| 10.0 | —d | — | |
| Abelcet | 1.0 | 2 ± 0.2b | 23 ± 3b |
| 10.0 | 9 ± 0.4 | 86 ± 6 | |
| LC-AMBc | 1.0 | 16 ± 4 | 178 ± 36b |
| 10.0 | 50 ± 7 | 467 ± 56 | |
| Lipids (DMPC/DMPG, 7/3) | 1.0 | 2 ± 0.3b | 18 ± 3b |
| LC-AMB lipid equivalentc | 10.0 | 10 ± 0.6 | 73 ± 4 |
| Mixed lipids (DMPC/DMPG, 7/3) | 1.0 | 24 ± 6 | 281 ± 19 |
| LC-AMB lipid equivalentc + AMBc | 10.0 | — | — |
a
Macrophages (105 per well) were cultured with various concentrations of AMB with IFN-γ (20 IU/ml). After an activation phase of 24 h, levels of nitrite and TNF-α production were determined as described in Materials and Methods. Results are means ± standard deviations of three measurements.
b
Significant difference from results with AMB by Student's t test (P > 0.05).
c
Prepared by a solvent displacement method.
d
—, amount was toxic.