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. 2022 Apr 4;14(4):788. doi: 10.3390/pharmaceutics14040788

Figure 6.

Figure 6

Confocal microscopy analysis of MDA-MB-231 (CD44+) cells treated with JE22-NPs (5). CD44-labeled cells treated with JE22-NPs (5) fluorescently labeled with Cy5 were analyzed (C) (Scale bar: 5 µm). The cells were incubated for 30 min with 1000 NPs/cell. Untreated cells (A) and cells labeled with anti-CD44-FITC (B) were used as controls (Scale bar: 5 µm). Images with an increase of 63× show a composition of the three channels used: blue, DAPI for the nucleus; green, FITC for anti-human CD44; and red, APC for JE22-NPs (5). (D) Confocal microscopy orthogonal (xy, xz, and yz) view representing the planes of intersection at the position of green cross line. The maximum intensity projection of the z-stack is shown blue for nuclei (DAPI), green for anti-human CD44 (FITC), and red for JE22-NPs (5) (APC).