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. 2022 Apr 4;14(4):788. doi: 10.3390/pharmaceutics14040788

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of the HA–CD44 interaction by flow cytometry. (A) Mean fluorescence of MDA-MB-231 cells analyzed by flow cytometry after treatment for 30 min at 4 °C with JE22-NPs (5), JE22, and anti-CD44 followed by incubation with HA-FITC for 15 min at 4 °C. Cells treated with unlabeled HA and HA-FITC were used as the negative and positive control, respectively. Error bars: ±SD from n = 3; p < 0.01, **; p < 0.001, *** (ANOVA). (B) Flow cytometry overlay histograms of HA-FITC competitive assay binding to MDA-MB-231 cells.