Figure 1.

Intracranial cerebrospinal fluid (CSF) after severe traumatic brain injury contains EVs, lipoproteins, and albumin. CSF samples from three patients (Pt) collected during the first three days (d) after severe traumatic brain injury were pooled in equal volumes to make a CSF sample (CSF-pool) for size exclusion chromatography (SEC). Individual CSF samples and the CSF-pool were separated by electrophoresis and analysed by immunoblot with antibodies against albumin, apolipoprotein (Apo) E and AI, and EV proteins CD81 and CD9. Sizes of detected proteins are indicated in kilodaltons.