Table 2.

Design and performance of four size exclusion chromatography (SEC) methods based on gravity flow and applied to separate cerebrospinal fluid from patients with severe traumatic brain injury.

Gravity Flow SEC	Column Packing Required	Average Flow Rate in mL/min	Volume of Mobile Phase Required for Elution of
			Nanoparticles	Total Proteins
Superose 6PG	yes	0.2	26 mL	68 mL
Sephacryl S-400	yes	0.3	28 mL	68 mL
Sepharose CL-6B	yes	0.6	25 mL	70 mL
qEV10/70 nm	no	3.6	26 mL	>74 mL