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. 2022 Apr 12;19(8):4640. doi: 10.3390/ijerph19084640

Table 1.

Half-lethal concentration (LC50) of Chlorpyrifos (CPF) in female African catfish, Clarias gariepinus.

CPF Concentration (µg/L) No. of Fish/Concentration No. of
Alive Fish
No. of
Dead Fish
a b a × b
0.00 8 8 0 0.0 0.0 0.00
25.00 8 6 2 25 1.00 25
50.00 8 4 4 25 3.00 75
100.00 8 2 6 50 5.00 250
200.00 8 1 7 100 6.50 650
400.00 8 0 8 200 7.50 1500
∑ a × b = 2500

Half-lethal concentration (LC50) of chlorpyrifos = the highest concentration − ∑a × b/n; a: constant factor of difference between groups; b: mean value of dead fish between every two successive groups; and n: number of fish in each group.