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. 2022 Mar 30;11(4):466. doi: 10.3390/antibiotics11040466

Figure 1.

Figure 1

The HS01 isolate carries a MDR plasmid, pCjH01. PFGE-S1 hybridization with specific probes for tet(O) and aphA-3 (aph(3′)-IIIa). Genomic DNA from the C. jejuni 81-176 strain carrying the pTet plasmid and the MDR H01 isolate was S1 digested, and linearized plasmids were separated by PFGE. The DNA was visualized by ethidium bromide (EtBr) staining and the presence of the tet(O) and aphA-3 genes was detected by hybridization using specific probes, as indicated. Lane 1, molecular mass marker (CHEF DNA Size Standard, 48.5–1000 kb, Lambda Ladder, Bio-Rad). The size of some of the bands in kilobases is indicated on the left side of the EtBr panel. Lanes 2 and 3, S1 digested genomic DNA from 81-176 and H01, respectively.