Table 1.
ORFs annotated in the plasmid pCjH01. The pCjH01-specific ORFs, as compared to pTet, are highlighted (grey background).
| Gene (ORF) | AA(MW) | Putative Function * |
|---|---|---|
| tetO | 639 (72.5) | Tetracycline resistance ribosomal protection protein |
| cpp2 | 57 (6.7) | Conjugal transfer protein |
| g1828 | 221 (25.5) | Relaxase/mobilization nuclease-domain-containing protein |
| cpp3 | 190 (22.1) | Hypothetical protein |
| aadE | 206 (24.3) | Aminoglycoside 6 adenyltransferase. |
| sat4 | 113 (13.4) | Streptothricin acetyltransferase |
| g1778 | 209 (23.9) | Aminoglycoside phosphotransferase |
| aphA-3 | 264 (31) | Aminoglycoside O phosphotransferase APH(3′)IIIa |
| g1780 | 221 (25.1) | Anti sigma factor (Zinc-finger domain containing protein) |
| g1781 | 150 (17.1) | Sigma 70 family RNA polymerase sigma factor |
| cpp6 | 58 (7.0) | Hypothetical protein |
| cpp7 | 96 (11.9) | Hypothetical protein |
| cpp8 | 117 (13.4) | Hypothetical protein |
| cpp9 | 170 (19.6) | Hypothetical protein |
| cpp10 | 185 (22.1) | Hypothetical protein |
| cpp11 | 88 (10.6) | Hypothetical protein |
| cpp12 | 186 (21.5) | ParA hypothetical protein |
| cpp13 | 88 (10.2) | Hypothetical protein |
| cpp14 | 1932 (224.3) | DEAD/DEAH box like helicase |
| g1790 | 314 (37.3) | HTH transcriptional regulator, XRE family |
| cpp17 | 462 (54.0) | Relaxase/mobilization domain containing protein |
| cpp18 | 183 (21.1) | Hypothetical protein |
| cpp19 | 93 (11.5) | Hypothetical protein |
| cpp20 | 203 (23.9) | NTPase. Hypothetical protein |
| cpp21 | 217 (26.0) | Hypothetical protein |
| traC | 408 (47.1) | DNA primase, TraC family |
| cpp23 | 87 (9.7) | EexN family lipoprotein. IncN-type entry exclusion |
| virB6 | 32 (3.6) | Truncated VirB6 protein |
| g1799 | 85 (10.2) | Hypothetical protein |
| g1832 | 61 (7.4) | Hypothetical protein |
| cpp24 | 72 (8.1) | Type-II toxin-antitoxin system HicB family antitoxin |
| cpp25 | 67 (7.9) | Type-II toxin-antitoxin system HicA family toxin |
| cpp26 | 597 (69.0) | AAA family ATPase |
| cpp27 | 204 (26.6) | Recombinase family protein |
| vapD | 125 (15.0) | Virulence associated protein |
| cpp29 | 107 (12.6) | Hypothetical protein |
| virB2 | 87 (9.2) | TrbC/VirB2 family protein. Conjugal transfer protein TraC |
| virB4 | 992 (106.3) | VirB4 family type IV secretion/conjugal transfer ATPase |
| cpp32 | 188 (21.8) | Rha family transcriptional regulator/phage regulatory protein |
| cpp33 | 221 (25.6) | Hypothetical protein |
| ssb1 | 141 (15.8) | single-stranded DNA-binding protein |
| cpp35 | 91 (10.7) | Hypothetical protein |
| virB5 | 323 (37.2) | Type IV secretion system protein |
| virB6 | 332 (35.5) | Type IV secretion system protein. TrbL/VirB6 family protein |
| virB7 | 56 (6.3) | Hypothetical protein |
| virB8 | 220 (25) | Type IV secretion system protein |
| virB9 | 295 (34.1) | P-type conjugative transfer protein VirB9 |
| virB10 | 391 (43.1) | Type IV secretion system protein VirB10. TrbI/VirB10 family protein |
| virB11 | 330 (37.6) | P-type DNA transfer ATPase VirB11 |
| virD4 | 603 (63.7) | Type IV secretory system conjugative DNA transfer family protein |
| cpp44 | 145 (16.8) | cag pathogenicity island protein |
| cpp45 | 254 (29.4) | Hypothetical protein |
| cpp46 | 265 (30.6) | Hypothetical protein |
| g1831 | 30 (3.9) | Hypothetical protein |
| cpp47 | 206 (23.8) | Hypothetical protein |
| topB | 730 (84) | DNA topoisomerase III |
| cpp50 | 473 (57) | Hypothetical protein |
| cpp51 | 59 (7.1) | Hypothetical protein |
* The predicted amino acids were used to search for putative function using the NCBI BLAST service with default blastp parameters.