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Clonogenic assay illustrating PC3 relative colony survival with varying doses of SPH, bicalutamide (BIC), or co-treatment with SPH and BIC. Treatments were applied daily for 5 days without changing the media. Post-12-day culturing cells were quantified with an automatic cell counter. p values less than 0.01 are summarized with two asterisks, and p values less than 0.001 are summarized with three asterisks.
