Table 1.

Gene expression assay results showing the effect of varying doses of monotreatment with bicalutamide (BIC) and co-treatment with BIC and SPH on the gene expression of FTH1 and TFRC genes in LNCaP and PC3 cells. Significant (>2-fold) up-regulation of FTH1 gene expression was observed in both LNCaP and PC3 cells. Significant (>2-fold) down-regulation of TFRC gene expression was observed in both LNCaP and PC3 cells.

Cell Line	Treatment	Gene	Fold Change			Average	SD
LNCaP	1 µM BIC	FTH1	1.0	1.1	1.0	1.0	0.1
	1 µM BIC	TFRC	1.0	1.0	1.2	1.1	0.1
LNCaP	40 µg/mL + 1 µM BIC	FTH1	2.3	2.2	2.3	2.3	0.1
	40 µg/mL + 1 µM BIC	TFRC	0.5	0.6	0.5	0.5	0.1
LNCaP	160 µg/mL + 1 µM BIC	FTH1	2.8	3.0	2.7	2.8	0.2
	160 µg/mL + 1 µM BIC	TFRC	0.3	0.4	0.5	0.4	0.1
PC3	10 µM BIC	FTH1	0.9	1.0	1.2	1.0	0.2
	10 µM BIC	TFRC	1.0	1.1	1.2	1.1	0.1
PC3	40 µg/mL + 10 µM BIC 40 µg/mL + 10 µM BIC	FTH1 TFRC	2.2	2.4	2.5	2.4	0.2
			0.5	0.5	0.4	0.5	0.1
PC3	160 µg/mL + 10 µM BIC 160 µg/mL + 10 µM BIC	FTH1 TFRC	2.8	2.6	2.4	2.6	0.2
			0.3	0.3	0.4	0.3	0.1