Explant area, number of neurites, and number of guided neurites per condition (without medium change). (A) The area of the cultured explants does not vary significantly between the experimental groups. (B) The mean number of neurites per 100,000 µm2 explant area independent of the direction of outgrowth is significantly increased in the NT-3 group compared to the NC, to the NP, and to CNTF. (C) The mean numbers of grown neurites dependent on the direction (+ or −) per 100,000 µm2 explant area of the NC, CNTF, and NT-3 groups are plotted. No guided neurite outgrowth is detectable. The mean number of neurites in the NT-3 + group is significantly higher than in the NC +. Additionally, a significant increase is visible between NT-3 − and NC −.