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. 2022 Apr 17;12(4):589. doi: 10.3390/biom12040589

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Neurite coverage area with and without directional analysis (without medium change). (A) The area covered with regenerated neurites is determined by marking the outline of the outgrown neurites and subtracting the respective spiral ganglion explant area. The outgrowth area independent of the direction is significantly increased in the NT-3 group compared to NC, NP, and CNTF. (B) Mean neurite outgrowth areas dependent on direction are plotted. A significant increase of the area in the NT-3 + group compared to NC + and to CNTF + is observed, whereas no differences are detectable within the groups.