Explant area, number of neurites, and number of guided neurites per condition with medium change (MC). (A) No differences in spiral ganglion explant (SGE) area of the groups with and without MC are detected. (B) The number of neurites per 100,000 µm2 SGE area independent of the orientation does not differ within the MC groups. In contrast, significantly more neurites grow in the NT-3 group without MC compared to the NC and the NP. (C) In this chart, the mean numbers of neurites grown towards (+) or opposed to the osmotic pump (−) per 100,000 µm2 SGE area of the NT-3 groups with and without MC are compared. No significant changes are observed.