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. 2022 Apr 17;12(4):589. doi: 10.3390/biom12040589

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Neurite length and coverage area with and without directional analysis with medium change (MC). (A) Mean neurite lengths of NT-3 and NT-3 MC independent of the direction are illustrated. NT-3 shows a significantly higher length compared to NT-3 MC. (B) Mean neurite lengths of NT-3 and NT-3 MC dependent on the direction of outgrowth are depicted. Compared to NT-3 MC +, the neurites of NT-3 + and NT-3 MC − are significantly longer. (C) The neurite coverage area independent of the direction of outgrowth does not differ between NT-3 and NT-3 MC. (D) The mean neurite outgrowth areas dependent on the direction show no significant differences between the NT-3 groups with or without medium change.