Figure 5.

Loss of VZV TK and TS affect virus sensitivity to select antivirals in cell culture. Cell-free VZV was added to ARPE-19 monolayers at 37 °C and compounds were added at 2 h postinoculation (HPI). Antiviral compounds remained in culture medium until VZV yield was measured on DPI 3. Infected cells were treated with cidofovir (CDV) and brivudine (BVdU) (A) in a range of concentrations, or (B) at specific concentrations of BVdU (1.25, 2.5, or 5 nM). VZV yield was calculated as the average Total Flux of treated wells divided by the average Total Flux of untreated wells. (A) Each point represents the mean Total Flux with the best fit line. (B) Each bar represents the mean + SD. Asterisks indicate significance between VZV viruses for each treatment condition (ns = no significance; ** p < 0.01; *** p < 0.001; **** p < 0.0001). p < 0.05; one-way ANOVA; Tukey post hoc test. n = 3 (A) to 6 (B) biological replicates.