Table 1.
Primers used to generate VZV viruses.
| Gene Target | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| fLuc Firefly (Photinus pyralis) Luciferase |
GAGGGATCCGGTTCCGGAGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGCCCAATGGAAGACGCC AAAAACATA | AATTCGAATTCGCGCGCAGATCTTTACACGGCGATCTTTCCGCCCTTCTTGGC |
| Zeo (Zeomycin resistance) |
AGATCTAGATCTCGAGTAATGGAACGGACCGTGTTGAC | GCTGACGTCGACGAATTCTGATCACTCAAGTTTCGAGGTCGAGGTG |
| gCLuc (F2 and R3; ORF14) |
CTTATCGCAGTTATCGCAACCCTATGCATCCGTTGCTGTTCAATGGACGAGCTGTACAAG | AATAAAATGATATACACAGACGCGTTTGGTTGGTTTCTGTCAGTCCTGCTCCTCAGCC |
| ORF9Luc (F2 and R3) |
AGTAGGGCCCGTTCGGCATCAAGAACTGATGCGCGAAAATCAATGGACGAGCTGTACAAG | ACGTTTATTTATTATACATAATACCGGGTAAACCGTTACTTCAGTCCTGCTCCTCAGCC |
| ORF17Luc (F2 and R2) |
CTCCACTCCCACTAAACACTGTATTAACAAAATATTGGAATTCAATGGACGAGCTGTACAAG | AGCAAAATAAAACAATGAACCATTAAGTCGCTCTTATGTGTGTCAGTCCTGCTCCTCAGCC |
| 57Luc (ORF57) |
ACGTTGAGGAGCCTTGCAGGTTGGGTGCCGCGCTTCACCGTCAATGGACGAGCTGTACAAG | TTTATATTTAACGGCTTTTAATTTGAAGACACCTATCCTCTCAGTCCTGCTCCTC |
| 40REP (ORF40) |
ATATAGATATTACGTTTATCATGCCAATGGGAGTGTTTCAGGCGAATTCCATGGACAGATATACACGACAGGATGACGACGATAAGTAGG | CAGTTGAAAAATCGCCGGCGTGTCGTGTATATCTGTCCATGGAATTCGCCTGAAACACTCCCATTGGCACAGGGTAATGCCAGTGTTAC |
| 50REP (ORF50) |
TGAATTATCCAAATTCGCCAATTAAGCGTATCCATTTGATGATCTAAAGCTTCCACCTCGGGTGTCGTGGTGTCGTACGGGGATGACGACGATAAGTAGG | TCTGAAAAAGTCTCACCGTACGACACCACGACACCCGAGGTGGAAGCTTTAGATCATCAAATGGATACGCTTAATAGGGTAATGCCAGTGTTAC |
| TS (TS T2B-For and endR2) |
TATCAAGTGGTCGTTTGTATTTAACGATTATTACCGGTACCGGTACCATGGTGAGCAAGGGCGAGGAG | ACATCTACTGTCTTGACAACATTTAAAAATCCATTAAAGATTATCTGGATCCTACCTTTC |
| TK (TK T2BFor and endR3) |
CGCCGAAGAATTTTTACACCACTTTGCAATAACACCAAACGGTACCATGGTGAGCAAGGGCGAGGAG | TGTGTATCATCTTTTTACTGGTACATACGTAAATACTAGGTTATCTGGATCCTACCTTTC |
| TKkan-del (For and Rev) | CGCCGAAGAATTTTTACACCACTTTGCAATAACACCAAACGGATGACGACGATAAGTAGG | TGTGTATCATCTTTTTACTGGTACATACGTAAATACTAGGAGGGTAATGCCAGTGTTAC |
The restriction sites engineered to sequences to enable cloning, as detailed in the text, are underlined.