Table 2.

Spurious mutations detected in pOka-DX BAC and their corrections.

Location of Mutation	Identification of Mutation and the Correction
ORF40 mutation in pOka DX	72661 atg cca atg gga gtg ttt cag Aca aac tcc atg gac aga tat aca cga cac …  M   P   M   G   V   F   Q  T428  N   S   M   E   R   Y  T   R   H
ORF40 Correction in pOka DX-RR	72661 atg cca atg gga gtg ttt cag GcG aaT tcc atg gac aga tat aca cga cac …  M   P   M   G   V   F   Q  A428  N   S   M   E   R   Y   T   R   H
ORF50 mutation in pOka DX	gaa aaa gtc tca ccg tac gac acc acg aca ccc gag gCg gaa gcg tta gat cat caa atg E11  K   V   S   P   Y   D   T   T   T   P   E   A   E   A   L   D   H   Q   M
ORF50 correction in pOka DX-RR	gaa aaa gtc tca ccg tac gac acc acg aca ccc gag gTg gaa gcT tta gat cat caa atg E11  K   V   S   P   Y   D   T   T   T   P   E   V   E   A   L   D   H   Q   M

Note: Lower case letters denote the sequence identical to the parent, while capital letters represent the spurious mutation in pOka DX (bolded), or the changed residues made to accompany the correction to introduce a novel restriction site (underlined). The single letter amino acid code of the region of the protein affected is shown below each sequence, with the key mutant residue bolded.