Fig. 3. Expression dynamics of miRNA in AML state-space.

(A) Hierarchical clustering of correlated miRNA expression annotated with state-transition critical point–based DE miRNAs. Four distinct patterns of miRNA expression are identified. (B) Four patterns, or groups of miRNA expression dynamics include monotonic (groups 2 and 4) and nonmonotonic (groups 1 and 3) patterns when plotted in the state-space (PC1). These patterns were discovered by fitting the average log-scaled expression for each sample in each expression group when plotted as a function of the PC1 coordinate of each sample. A local maximum (group 1) and minimum (group 3) are identified near the unstable transition critical point c₂. (C) Pathways implicated by the nonmonotonic expression pattern in group 1 include cell/nuclear signaling pathways including IL-6, TNFα-NFκB, cytokine inflammation response, and Wnt signaling. The opposite expression pattern (group 3) is enriched for immune response pathways, including antigen processing and TLR signaling (complete list provided in table S7.)