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. 2022 Apr 16;10(4):828. doi: 10.3390/microorganisms10040828

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Maps of the plasmids constructed in this study. All three plasmids are replicative in E. coli. pEA01 can replicate in Amycolatopsis while pEA02 and pEA03 are suicide vectors in Amycolatopsis. aph(3′): kanamycin resistance gene; pBR-ori: pBR322 origin of replication; pA-rep: short replicon region of pA387; pac: puromycin resistance gene; oriT: origin of transfer; int and xis, integrase and excisionase gene of pSAM2; trc_p: trc promoter for the expression of xis and int genes; ermE: erythromycin resistance gene; attL: left attachment site; ColE1: ColE1 origin of replication; aac(3)IV: apramycin resistance gene; aph(7″): hygromycin resistance gene; p15A: p15A origin of replication; attR: right attachment site.