Table 1.
Strains used in this study.
| Strain | Description | Reference or Source |
|---|---|---|
| E. coli DH5α | General cloning strain | Promega |
| E. coli ET12567/pUZ8002 | Host strain for conjugation from E. coli to Amycolatopsis | [30,31] |
| E. coli ET12567/pUZ8003 | Host strain for conjugation from E. coli to Amycolatopsis (pUZ8003 is a modified pUZ8002 with aph(3′) gene replaced by bla) | [32] |
| A. mediterranei DSM 40773 | Wild-type (WT) strain | DSMZ |
| DSM 40773 ∆rifK::att1Ωhyg | A. mediterranei rifK deletion mutant with replacement of the rifK gene by the att1Ωhyg hygromycin resistance cassette | This study |
| DSM 40773 ∆rifK::att1 | Unmarked A. mediterranei rifK deletion mutant | This study |
| DSM 40773-pRIF14 | A. mediterranei containing pRIF14 | This study |
| DSM 40773-pRIF12-pRIF14 | A. mediterranei containing both plasmids pRIF14 and pRIF12 | This study |
| DSM 40773 ∆rif::att1 | A. mediterranei with a deletion of the complete rif cluster | This study |
| S. aureus HG003 | Rifamycin sensitive strain used as indicator in bioassay analysis | [33] |
| S. aureus HG003 rpoB (H418Y) | Rifamycin resistant strain used as indicator in bioassay analysis, rpoB mutant of S. aureus HG003 | Marick Esberard and Philippe Bouloc unpublished |