Figure 1.

Confocal micrograph of cultured neurons, showing a conventional distribution of different tubulin post-translational modifications along the axon (arrowhead) and inside an enlarged terminal (arrow). Tyrosinated tubulin (Tyr TUB, red) is present in the axon and constitutes the microtubule network at the terminal; acetylated tubulin (Ac TUB, green) is enriched along the axon and barely present at the tip; Δ2 tubulin (Δ2 TUB, white) is detectable only in the axon shaft showing a proximal-to-distal distribution. Scale bar = 15 µm.