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. 2022 Apr 8;11(8):1262. doi: 10.3390/cells11081262

Figure 1.

Figure 1

SMYD2 deficiency decreases TMPRSS2 expression in HT-29 cells. (A) Immunoblot analysis of SMYD2 in lysates of WT and two clones of CRISPR/Cas9-directed SMYD2 knockout HT-29 cells. β-actin served as a loading control. (B) The workflow of RNA-seq analysis on HT-29 xenografts. (C) RNA-seq analysis of TMPRSS2 expression (normalized counts) in HT-29 xenografts. (D) qPCR analysis (Left panel) or immunofluorescence staining (Right panel) of TMPRSS2 in cultured WT and SMYD2 knockout HT-29 cells. Experiments were performed twice and representative data are shown. *** p < 0.001. Scale bar, 10 µm.