Antigenic, allergenic and physicochemical characteristics of the construct.
| Characteristics | Finding | Remarks |
|---|---|---|
| Number of amino acids | 575 | Suitable |
| Molecular weight | 62 355.33 Da | High |
| Theoretical pI | 9.91 | Basic |
| Chemical formula | C2883H4445N747O773S13 | — |
| Extinction coefficient (at 280 nm in H2O) | 118 860 M−1 cm−1 | — |
| Estimated half-life (mammalian reticulocytes, in vitro) | 30 hours | — |
| Estimated half-life (yeast-cells, in vivo) | >20 hours | — |
| Estimated half-life (E. coli, in vivo) | >10 hours | — |
| Instability index of vaccine | 31.26 | Stable |
| Aliphatic index of vaccine | 76.47 | Thermostable |
| Grand average of hydropathicity (GRAVY) | −0.139 | Hydrophilic |
| Antigenicity | 0.6153 | Antigenic |
| Allergenicity | No | Non-allergen |
| Solubility | 0.701681 | Soluble |