The thermodynamic parameters (stoichiometry, K_a, ΔH, ΔS, and ΔG) derived from ITC as proteins (βlg and MB) were titrated into AuNPs at different temperature.

Protein	Temperature (°C)	Stoichiometry (103)	K a a (105 M−1)	ΔH (105 J M−1)	ΔSb (103 J M−1 deg.)	ΔGb (104 J)
βlg	4	3.9	34 ± 10	−39 ± 12	−13.9	−3.5
	24	3.1	29 ± 7	−32 ± 9	−10.6	−3.7
	44	2.6	25 ± 6	−29 ± 7	−9.0	−3.9
MB	4	3.6	2.6 ± 0.7	−4.9 ± 0.7	−1.6	−2.9
	24	3.0	2.3 ± 0.4	−4.6 ± 0.8	−1.4	−3.0
	44	2.6	2.1 ± 0.5	−4.3 ± 0.5	−1.2	−3.2

^aDerived from one site binding model.

^bCalculated according to the eqn (1) and (2).