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. 2021 May 25;11(31):18915–18929. doi: 10.1039/d1ra01411k

Independent variables and CCD coded levels for the hydrolysis of DORB protein.

Independent variables Code levels
−1.68 −1 0 1 1.68
X 1: temperature (°C) 41.59 45.00 50.00 55.00 58.41
X 2: E/S (% w/v) 0.50 0.90 1.50 2.10 2.50
X 3: hydrolysis time (min) 79.09 120.00 180.00 240.00 280.91