Independent variable levels for temperature (X₁), enzyme (X₂), and time (X₃), with tested values of the two response variables: % DH (Y₁), and lipase inhibition (IC₅₀; μg mL⁻¹) (Y₂)^a.

Run	Space type	Coded levels			Independent variables			Response
		X 1	X 2	X 3	X 1	X 2	X 3	Y 1	Y 2
1	Factorial	−1	−1	−1	45.00	0.90	120.00	25.03	9.77
2	Factorial	+1	−1	−1	55.00	0.90	120.00	30.10	14.33
3	Factorial	−1	+1	−1	45.00	2.10	120.00	31.23	11.00
4	Factorial	+1	+1	−1	55.00	2.10	120.00	33.91	6.30
5	Factorial	−1	−1	+1	45.00	0.90	240.00	30.56	7.06
6	Factorial	+1	−1	+1	55.00	0.90	240.00	31.74	14.69
7	Factorial	−1	+1	+1	45.00	2.10	240.00	36.13	19.25
8	Factorial	+1	+1	+1	55.00	2.10	240.00	36.47	19.68
9	Axial	−1.68	0	0	41.59	1.50	180.00	29.34	15.92
10	Axial	+1.68	0	0	58.41	1.50	180.00	34.64	18.01
11	Axial	0	−1.68	0	50.00	0.50	180.00	27.83	9.19
12	Axial	0	+1.68	0	50.00	2.50	180.00	39.79	12.50
13	Axial	0	0	−1.68	50.00	1.50	79.09	30.15	7.31
14	Axial	0	0	+1.68	50.00	1.50	280.91	34.93	12.98
15	Center	0	0	0	50.00	1.50	180.00	34.54	3.02
16	Center	0	0	0	50.00	1.50	180.00	35.24	3.83
17	Center	0	0	0	50.00	1.50	180.00	34.36	4.12
18	Center	0	0	0	50.00	1.50	180.00	34.51	3.46
19	Center	0	0	0	50.00	1.50	180.00	33.07	3.65
20	Center	0	0	0	50.00	1.50	180.00	34.89	3.89

^aAll tests were performed in triplicate.