Comparison between validation parameters of the proposed TLC-visualization method and TLC-densitometric method for determination of ofloxacin and ornidazole in their binary mixture.
| TLC-visualization method | TLC-densitometric method | |||
|---|---|---|---|---|
| Parameters | OFL | ORN | OFL | ORN |
| Wave length | 320.0 nm | 320.0 nm | ||
| Range (μg/band) | 12.5–62.5 | 500–1000 | 5–40 | 5–50 |
| Linearity (regression equation) | Y = −0.1466x + 16.183 | Y = −0.0171x + 43.553 | Y = 434.5x + 10 455 | Y = 516.98x + 23 107 |
| Slopea | 0.15 | 0.02 | 434.5 | 516.9 |
| Intercepta | 16.2 | 43.6 | 10 455 | 23 107 |
| Correlation coefficienta | 0.9999 | 0.9991 | 0.9999 | 0.9999 |
| Accuracyb (mean ± SD) | 100.0 ± 0.6 | 100.1 ± 0.7 | 99.9 ± 0.7 | 99.7 ± 0.9 |
| Specificityc | 98.6 ± 0.4 | 100.5 ± 1.5 | ||
| LOD (μg/band)d | 1.6 | 97.8 | 0.8 | 1.1 |
| LOQ (μg/bandd | 4.9 | 296.3 | 2.3 | 3.3 |
| Precision (±RSD%) | ||||
| (a) Repeatabilitye | ±0.8 | ±0.7 | ±0.4 | ±0.8 |
| (b) Intermediate precisionf | ±0.4 | ±0.5 | ±0.3 | ±0.7 |
Average of three determinations.
Accuracy (the mean of 5 different concentrations of each OFL and OR).
Recovery of different laboratory prepared mixtures containing different ratios of OFL and OR.
Limit of detection is determined via calculations, LOD = (SD of response/slope) × 3.3; LOQ = (SD of response/slope) × 10.
Intraday precision (the RSD of 3 different concentrations) (10, 20, 25 μg/band for OFL) & (5, 10, 50 μg/band for OR) in (TLC-densitometric) and (12.5, 25, 37.5 μg/band for OFL) & (500, 700, 900 μg/band for OR) in TLC-smartphone, 3 replicates each, within the same day.
Interday precision (the RSD of 3 different concentrations) (10, 20, 25 μg/band for OFL) & (5, 10, 50 μg/band for OR) in (TLC-densitometric) and (12.5, 25, 37.5 μg/band for OFL) & (500, 700, 900 μg/band for OR) in TLC-smartphone, 3 replicates each, on 3 successive days.