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. 2022 Mar 24;24(4):565–578. doi: 10.1038/s41556-022-00866-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Macroscopy of representative human metastasis-free lymph node (MFLN) (top) and follicular lymphoma (FL) (bottom) samples. Scale bars, 1 cm. b, Gating strategy for the isolation and analysis of non-haematopoietic cells (NHCs) in human LN and lymphoma in flow cytometry (see ‘Single-cell isolation of LNNHCs’ in the Methods section). BEC, blood endothelial cell; LEC, lymphatic endothelial cell; NESC, non-endothelial stromal cell; scRNA-seq, single-cell RNA sequencing. c, Overview of scRNA-seq analysis conducted in the present study. PTCL, peripheral T-cell lymphoma; tDLBCL, diffuse large B-cell lymphoma transformed from FL. d, UMAP plots of NHCs, colour coded by patients (top left), sites of sample collection (top right), and patient age (bottom), according to patient cohorts. e, Proportion of each major NHC component between MFLN (left) and FL (right) cohorts. f, PDPN expression in stroma-enriched cells from MFLN samples. High magnification image indicates heterogeneous PDPN expression levels among LECs. g, Strategy used to detect BECs (top), LECs (middle), and NESCs (bottom) using flow cytometry (FCM, red-coloured) or scRNA-seq (red circles) analysis in a representative case (MFLN 8).