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Scheme for the synthesis of 2TA esters for the in vitro 2TA–terbium luminescence assay. (A) 2-Thiopheneacetic acid (1) is readily converted into esters through condensation with alcohols either via boiling in the alcohol with sulfuric acid (Fischer esterification) or through the use of DCC and DMAP. (B) Compounds that influence the luminescent response of ester cleavage.
