Fig. 1.

SMARTseq2 reveals strong transcriptional heterogeneity of single ITSCs in their NCSC-marker expression. A: ITSCs were derived from the inferior turbinate of the human nasal cavity. B: GFP-transfected ITCSs 24 h after transfection. C: Flow cytometric analyses of ITSCs. SSC and FSC measure size and granularity of the cells. Whole cells (44.1 %) are marked for further gating. D: Sorting of GFP⁺ ITSCs. FL2 measures emitted fluorescence signal (GFP). Untransfected cells served as negative control. E: RT-PCR after SMARTseq2 for the housekeeping genes GAPDH, eEF2 and Vimentin as well as for the NCSC-markers S100, Snail and Twist. F-K: RT-PCRs after application of SMARTseq2 on manually diluted single cells from three male and three female donors for the housekeeping gene GAPDH and the stem cell and neural crest markers Nestin, Slug, Snail and Twist