Fig. 2.

Immunocytochemistry confirmed heterogeneous expression of Nestin and S100 between ITSCs in dependence to the donor. A: Schematic view on interpopulational heterogeneity in stem cell marker expression between individual ITSC-populations in dependence to the donor. B-C: Representative images of immunocytochemical stained Nestin protein in female and male-derived ITSCs. D: Quantification of immunocytochemistry via measurement of the cytosolic fluorescence intensity of Nestin per single cell validated a significant heterogeneity dependent on the donor on protein level. E-F: Representative images of immunocytochemical stained S100 protein in female and male-derived ITSCs. G: Quantification of immunocytochemistry via measurement of the cytosolic fluorescence intensity of S100 per single cell affirmed the heterogeneity of S100 expression on protein level. Kruskal-Wallis test, Post test: Dunn’s Multiple Comparison Test, *p<0.05, *p<0.01, *** p < 0.001. was considered significant