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. 2022 Jan 8;18(4):1434–1443. doi: 10.1007/s12015-021-10324-6
Diluted annealed oligonucleotides 3ul
ABE/CBE sgRNA expression plasmid 1ul
T4 DNA Ligase 1ul
10X T4 DNA ligase buffer 2ul
ddH2O 13ul