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. 2022 Apr 22;13:2206. doi: 10.1038/s41467-022-29905-1

Fig. 5. FSP1 inhibition sensitizes KEAP1 deficient lung cancer cells to radiation by inducing ferroptosis.

Fig. 5

a Clonogenic survival curves of the indicated H1299 cells exposed to X-ray irradiation at indicated doses. b, c Lipid peroxidation levels (b) and PTGS2 mRNA levels (c) in the indicated H1299 cells at 24 h after 6 Gy X-ray irradiation. d Clonogenic survival curves of the indicated H1299 cells exposed to X-ray irradiation at indicated doses. e, f lipid peroxidation levels (e) and PTGS2 mRNA levels (f) in the indicated H1299 cells at 24 h after 6 Gy X-ray irradiation. g Clonogenic survival curves of the indicated H1299 cells exposed to X-ray irradiation at indicated doses following pretreatment with the indicated doses of iFSP1 or DMSO for 24 h. h Lipid peroxidation levels in the indicated H1299 cells at 24 h after exposure to 6 Gy X-ray irradiation following pretreatment with iFSP1 or DMSO for 24 h. i Protein levels of FSP1 in A549 FSP1 KO cells. j Clonogenic survival curves of the indicated A549 cells exposed to X-ray irradiation at indicated doses. k Lipid peroxidation levels in the indicated A549 cells at 24 h after exposure to 6 Gy X-ray irradiation. l–n Clonogenic survival curves of A549 (l), H460 (m), and H2126 (n) cells exposed to X-ray irradiation at indicated doses following pretreatment with the indicated doses of iFSP1 or DMSO for 24 h. o Lipid peroxidation levels in A549, H460, and H2126 cells at 24 h after exposure to 6 Gy X-ray irradiation following pretreatment with iFSP1 or DMSO for 24 h. Data were presented as (if mentioned otherwise) mean ± SD; n = 3. P value was determined by a two-tailed unpaired Student’s t-test; ns not significant. Source data are provided as a Source Data file.