Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 22;13(4):398. doi: 10.1038/s41419-022-04741-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Images of mitochondrial network in sensitive (2008, U2OS) and resistant (C13, U2OS-PT) cells acquired by confocal microscopy Zeiss using TOM20 immunostaining, 488. Scale bar 20 µm. B Mitochondria segmentation was performed using the ImageJ Squassh plugin (Rizk et al., 2014); size and morphology features were measured by using Fiji. Data from 15 different cells per cell line. *p < 0.05, calculated by a two-tailed unpaired t-test comparing resistant vs sensitive cells. C Images of mitochondrial morphology in sensitive (2008, U2OS) and resistant (C13, U2OS-PT) cells acquired by Tecnai G2 (FEI) transmission electron microscope operating at 100 kV; images were collected by a F114 (TVIPS) CCD camera. The TEM images and experiment are performed by the University of Padova electron microscopy facility. Scale bar 1 µm and 500 nm. D The morphometric analysis was performed using ImageJ freehand tool (at least 5 cells per sample, at least 50 images/sample). Data are the mean ± SEM of three different experiments; **p < 0.01, ***p < 0.001, calculated by a two-tailed unpaired t-test comparing resistant vs sensitive cells. E, F Expression of OPA1, MFN1, MFN2, MFFs, p-DRP1 and total DRP1. G The optical density (O.D.) was normalized respectively to TOM20; for total DRP1 to β-ACTIN for 2008-C13 or calnexin for U2OS-U2OS-PT in cancer cells sensitive and resistant. The data are expressed as ratio of resistant cells to sensitive. Data are the mean ± SEM of 4–5 different experiments; *p < 0.05; **p < 0.01, ***p < 0.001, calculated by a two-tailed unpaired t-test comparing resistant vs sensitive cells. H mRNA expression of genes OPA1, MFN2, DRP1 and H-FIS1 normalized to β-actin for 2008-C13 or calnexin for U2OS-U2OS-PT. The data are expressed as a ratio of resistant cells to sensitive cells set to 1. Data are the mean ± SEM of 4–5 different experiments; *p < 0.05; **p < 0.01, calculated by a two-tailed unpaired t-test comparing resistant vs sensitive cells.