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. 2022 Apr 22;13(4):398. doi: 10.1038/s41419-022-04741-9

Fig. 4. BNIP3 silencing reduces mitochondrial mass loss.

Fig. 4

A 2008-C13 and U2OS-U2OS-PT were transfected with a non-targeting control (NTC, scramble) esiRNA or with an esiRNA targeting BNIP3 (esiBNIP3). BNIP3 protein expression levels were normalized to TOM20; in (B) the respective quantification. Data are the mean ± SEM of 5 different experiments. **p < 0.01, ***p < 0.001, calculated by a two-tailed unpaired t-test comparing esiRNA targeting BNIP3 vs non-targeting control cells. (C) and (E) Effect of 24 h of CDDP (1 µM) and Bafilomycin A1 (100 nM) treatment on mitochondrial proteins expression of 2008-C13 and U2OS-U2OS-PT transfected with non-targeting control (NTC, scramble) esiRNA or with an esiRNA targeting BNIP3 (esiBNIP3), and the respective quantification (D) and (F). The optical density of TOM20, Cyclophilin D and COX IV was normalized to β-ACTIN for ovarian cancer cells and to calnexin for osteosarcoma cells sensitive and resistant. The data are expressed as the ratio of treated cells respect to untreated cells; *p < 0.05, calculated by a two-tailed unpaired t-test comparing esiRNA targeting BNIP3 (esiBNIP3) vs non-targeting control (NTC, scramble) cells. Data are the mean ± SEM of 4–5 different experiments.