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. 2022 Apr 22;12:6619. doi: 10.1038/s41598-022-10605-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The results for experiment 2: Facilitation over repeated saccades and the saccade size. A fixation-related microsaccade (a) and saccade (from MS-free fixations) and (b) RTs as a function of the serial saccade number over time (X-axes) and saccade size (Y-axes). Light and dark colors represent longer and shorter RTs, respectively. 2D smoothing was applied (see the “Methods” section) and all conditions were combined for a clearer demonstration of the similar trend for msRT and sacRT. (c) A fixation-related saccade (blue)/microsaccade (gray) latency (sac/ms RT) as a function of saccade distance (deg), averaged across observers (n = 13) with 1SE error bars computed on the demeaned data (see the “Methods” section). Significance was assessed using LMM (see the “Methods” section). b(slope) = 4.9, t(118) = 3.77, p ≤ 0.0003 with CI (2.3, 7.5); b(slope) = 4.1, t(118) = 2.8, p ≤ 0.005 with CI (1.2, 7), for sacRT and msRT, respectively. (d) A fixation-related saccade (blue)/microsaccade (gray) latency (sac/ms RT) as a function of the serial saccade number, averaged across observers (n = 16) with 1SE error bars computed on the demeaned data (see the “Methods” section). Significance was assessed using LMM (see the “Methods”); b(slope) = − 4.9, t(126) = − 3.9, p ≤ 0.0001 with CI (− 7.3, − 2.4); b(slope) = − 3, t(118) = − 2.2, p ≤ 0.02 with CI (− 5.7, − 0.3), for sacRT and msRT, respectively.