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. 2022 Apr 22;12:6616. doi: 10.1038/s41598-022-10585-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative fluorescence histograms of PI-stained somatic and gametic cells of L. salmonis salmonis. (a) Run 1, somatic cells obtained from whole body squash of nauplii; (b) run 3, somatic cells obtained from cephalic regions of adult females; (c) run 4; oocyte and sperm samples; (d) run 5, somatic cells obtained from cephalic regions of adult males and females. Samples analyzed using chicken and human cells as internal reference standards. Fluorescence (FL) peaks values on the X-axis are reported in arbitrary units expressed as Propidium Iodide (PI) fluorescence channel numbers (FL value). Values on the Y-axis (counts) refer to the number of nuclei counted per channel. CEN chicken erythrocytes nuclei, MNCs human mono-nucleated cells.