Table 3.
Nuclear DNA contents of somatic and gametic cells of L. salmonis salmonis as measured using flow cytometry.
| Run | Origin | Stage | Tissue | N | 2C value (pg) vs. CEN Mean ± SEM |
2C value (pg) vs. MNCs Mean ± SEM |
CV (%) | 2C value (Gb) |
|---|---|---|---|---|---|---|---|---|
| 1 | LsTromsø | Nauplii | WB | 10 | 3.08 ± 0.009 | na | 2–4 | 3.01 |
| 2 | LsTromsø | Nauplii | WB | 11 | 3.09 ± 0.005 | 3.06 ± 0.021 | 2–3 | 3.01* |
| 3 | LsTromsø | Adult ♀ | CT | 3 | 3.10 ± 0.020 | 3.12 ± 0.008 | 3–5 | 3.04* |
| Adult ♂ | CT | 6 | 3.14 ± 0.139 | 3.14 ± 0.010 | 3–5 | 3.07* | ||
| 4 | LsGulen | Adult ♀ | O | 5 | 3.36 ± 0.003 | 3.31 ± 0.003 | 2–4 | 3.26* |
| Adult ♂ | S | 5 | 1.69 ± 0.006 | 1.68 ± 0.002 | 1–3 | 1.65* | ||
| 5 | LsTromsø | Adult ♀ | CT | 5 | 3.01b ± 0.022 | na | 2–3 | 2.94 |
| LsTromsø | Adult ♂ | CT | 5 | 3.19a ± 0.016 | na | 3–4 | 3.12 | |
| Wild | Adult ♀ | CT | 11 | 3.08b ± 0.031 | na | 2–3 | 3.01 | |
| Wild | Adult ♂ | CT | 5 | 3.19a ± 0.013 | na | 3–5 | 3.12 |
Runs 1, 2, 3, and 5 measured somatic cells of nauplii or adults of the LsTromsø strain. Run 4 measured gametic cells of the Ls Gulen laboratory strain. Run 5 compared the laboratory reared LsTromsø strain to wild caught adults from naturally infected fish reared in Tromsø. Chicken and/or human white blood cells were used as internal reference standards.
CEN Chicken erythrocyte nuclei (2C value = 2.5 pg DNA per nucleus), MNCs human mono-nucleated cells (2C value = 7.0 pg DNA per nucleus), WB whole body, CT cuticular and subcuticular tissues from cephalic region, O oocytes, S sperm, N number of individuals or number of samples in the cases of nauplii analyzed, CV coefficient of variation as a percentage of mean for target nuclei (L. salmonis data), Na not available. For naupliar stages (run 1 and 2), each sample consisted of approximately 50 nauplii.
*Average value based on the two internal standards. For Run 5, 2C values with superscripts “a” and “b” differ at P < 0.05 (two-way ANOVA).