Table 4.
Nuclear DNA contents of somatic cells of individual adult L. salmonis as measured using Feulgen image analysis densitometry.
| Origin | Stage | Tissue | N | 2C value (pg) vs. CEN Mean ± SEM |
2C value (pg) vs. MNCs Mean ± SEM |
CV (%) | 2C value (Gb) |
|---|---|---|---|---|---|---|---|
| Laboratory | |||||||
| Ls Gulen 61 | Adult ♀ | CT | 13 | 2.78 ± 0.012 | na | 1.6 | 2.74 |
| Ls Gulen 62 | Adult ♀ | CT | 15 | 2.64 ± 0.003 | na | 3.3 | 2.60 |
| Ls Gulen 63 | Adult ♀ | CT | 92 | 2.67 ± 0.003 | na | 10.4 | 2.63 |
| Average | 2.70 ± 0.043 | na | 2.66 | ||||
| Ls1a 133 | Adult ♀ | CT | 34 | 2.64 ± 0.046 | 2.60 ± 0.046 | 10.2 | 2.60 |
| Ls1a 134 | Adult ♀ | CT | 20 | 2.75 ± 0.037 | 2.71 ± 0.037 | 6.1 | 2.67 |
| Ls1a 137 | Adult ♀ | CT | 16 | 2.71 ± 0.025 | 2.67 ± 0.025 | 3.9 | 2.65 |
| Average | 2.70 ± 0.032 | 2.66 ± 0.032 | 2.66 | ||||
| Ls Gulen 64 | Adult ♂ | CT | 65 | 2.93 ± 0.035 | na | 9.9 | 2.89 |
| Ls Gulen 65 | Adult ♂ | CT | 36 | 2.86 ± 0.037 | na | 7.9 | 2.82 |
| Ls Gulen 66 | Adult ♂ | CT | 19 | 2.90 ± 0.054 | na | 8.3 | 2.85 |
| Average | 2.90 ± 0.020 | na | 2.85 | ||||
| Wild | |||||||
| Copscook Bay, Maine | Adult ♀ | SP | 3 | 3.07 ± 0.176 | na | 9.8 | 3.00 |
Cephalothorax (CT) tissues of laboratory reared adults were obtained from the Ls Gulen and Ls1a populations. Tissue from the spine of an appendage (SP) was obtained from a wild caught Maine population. Hen (CEN) and male human mononucleated leucocytes (MNCs) were used as internal reference standards to estimate values in picograms (pg). Values based on hen were converted to gigabases (Gb). N refers to number of nuclei measured in each adult. SEM refers to standard error of the mean; CV refers to coefficient of variation of IOD values.