Fig. 2. (a) Free ICG and ICG@PSMA NPs in aqueous dispersion under the irradiation of daylight (left) and in vivo fluorescence imaging system (right, λem = 800–1000 nm, λex = 745 nm). (b) The fluorescence imaging of free ICG and ICG@PSMA NPs in aqueous dispersion (200 μg mL−1, counted by ICG content) under the confocal fluorescence microscope (λem = 800–1000 nm, λex = 640 nm). (c) Cytotoxicity of HeLa cells after incubation with free ICG and ICG@PSMA NPs at different concentrations for 24 hours. Data are expressed as mean ± S.D. (n = 3 in each concentration).
